During the annual management of fruit trees, the fertilization work during fruit expansion is particularly important. At this time, the fruit has changed from a nitrogen nutrient period to a sugar nutrient period, requiring a large supply of nutrient water. If it is malnourished, it will lead to fruit splitting. Therefore, during the fruit expansion period, it is necessary to ensure sufficient water and nutrients to meet the physiological needs of fruit development, so that the fruit is swollen rapidly, the color is good, and the fruit drop and fruit cracking phenomenon are reduced. Improve fruit coloration, preserve fruit, strengthen fruit, and improve fruit quality. This edition of the manuscript sorts out the knowledge of fertilizer management in fruit expansion period, in order to help readers.
Before the apple fruit is inflated, nitrogen and phosphorus-based fertilizers are supplied to ensure the normal development of green organs, and more green photosynthetic tissues are formed. This period is called nitrogen nutrition period; once entering the expansion period, apples will be transformed from nitrogen nutrition period. During the sugar-to-nutrition period, the carbohydrates produced by photosynthesis of green organs are transported to the fruit for expansion. Therefore, during this period, attention should be paid to the supplement of nutrients such as potassium, calcium, magnesium and boron. While meeting the nutrient requirements of photosynthesis of green organs, it is also necessary to promote the transfer of these photosynthetic products into fruits as much as possible.
Potassium and potassium supplementation is not a component of plant organs, but it is an indispensable "character" in physiological activities such as nutrient synthesis, transport and transformation in plants. In this period, the use of sufficient potassium fertilizer can make the fruit expand better and taste. Better. As for the application rate of potash fertilizer, the apple tree with a yield of about 100 kg can be applied with about 2 kg of water-soluble good nitric oxide-based high-potassium compound fertilizer, and combined with sterilization and insecticide spraying on the surface of the plant for 2 to 3 times. 1000 times of high-quality potassium dihydrogen phosphate, root and leaf combined with nutrients to quickly and continuously replenish nutrients.
The amount of phosphorus supplemented with phosphorus and nitrogen is not only conducive to flower bud differentiation, but also conducive to the transport and transformation of organic nutrients. The supplement of nitrogen is a contradiction in this period. Insufficient nitrogen nutrition can easily lead to premature senescence of fruit trees and even deciduous fruit drop in advance. Nitrogen nutrition is too much, but it is easy to cause long leaves and leaves, which is not conducive to the flow of photosynthetic products to fruits, resulting in poor quality or even loss of fruit. Therefore, fertilization in this period should be based on the principle of fertilization and fertilization. For the weak trees with large amount of results, it is necessary to apply more fertilizers to the young trees with less results to promote the flower bud differentiation when the new shoots stop growing; "The tree should focus on topdressing before and after germination. In saline-alkali soils, the available phosphorus content in the soil is low, and phosphate fertilizer should be added during topdressing.
The demand for calcium fertilizer in the expansion period of calcium supplement apple accounts for 70% of the whole year. Calcium is an indispensable nutrient element for the development of fruit epidermis. The sufficient nutrient nutrition can not only reduce the occurrence of bitter pox disease, but also reduce fruit cracking and bright peel. Fruit quality and storage resistance are essential. You can choose chelating calcium fertilizer (currently there are fruit surface, leaf double-absorbed calcium glycol alcohol) diluted 800 ~ 1000 times liquid 2 ~ 4 times.
During the expansion period of magnesium supplementation, the nearby leaves with large fruit masses often see magnesium deficiency in different degrees of chlorosis between veins. It can be solved by spraying 800 to 1000 times of magnesium sulfate. Because magnesium supplementation enhances photosynthesis and chlorophyll content, ensuring the need for fruit enlargement.
Boron supplementation enters the fruit expansion period, and reproductive growth is strong. As an important nutrient element in this stage, boron plays an important role in the rapid increase of carbohydrate transport and fruit formation. If the nutrients are not replenished in the flowering period, 800-1000 times of high-quality boron fertilizer can be sprayed again. This top dressing can effectively accelerate the rapid expansion of the fruit and increase the cell expansion rate of the fruit. It plays an important role in improving production, overcoming the "years and years" of the tree and promoting flower bud differentiation.
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Nucleic Acid (DNA/RNA) Extraction Kit
1. Introduction
The total viral nucleic acid extraction kit is suitable for extracting total viral nucleic acid from serum, plasma, tissue homogenate and other samples. The kit is based on silica column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. This product has successfully extracted nucleic acids from hepatitis B A/C, hepatitis C, and norovirus standard. The obtained DNA/RNA can be directly used in a series of downstream experiments such as PCR, RT-PCR, and LAMP.
Notice:
1. The carrier RNA solid must be dissolved in Nuclease Free Water to 1µg/µl before use, and vortex to dissolve. Store in aliquots at -70°C. If you need to store it at -20℃ for a long time, please repackage it according to the number of times of use.
2. Dissolve Proteinase K (20mg/ml): Add Proteinase Dissolve Buffer to dissolve Proteinase K to a final concentration of 20mg/ml. Proteinase K dry powder can be stored at 2-8°C for one year, but dissolved Proteinase K must be stored in aliquots at -20°C. Repeated freezing and thawing of Proteinase K can affect its activity.
3. Buffer VHB must be diluted with 14 ml absolute ethanol before use and stored at room temperature.
4. Buffer RW2 must be diluted with 80 ml of absolute ethanol before use and stored at room temperature.
3. Shelf life
Except for Proteinase K and Carrier RNA, other components of this product can be stored at room temperature (15-25°C) for 12 months, and should be stored at 2-8°C for long-term storage. Proteinase K and Carrier RNA dry powder are transported at room temperature. Please store at -20°C after receiving the test product, and store at -20°C after dissolving.
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