Three cases concerning GNT beads can be recycled Analysis
Article source: Luoyang Jiente Biotechnology Co., Ltd.
Magnetic bead nucleic acid extraction has received more and more attention in the current biotechnology industry. Many companies have begun to transfer from traditional nucleic acid extraction methods to automated magnetic beads, and biomagnetic beads have naturally become the core of key technologies.
Recently, many teachers have asked if the biomagnetic beads of the GNT series can be recycled. Theoretically, the binding and release of the magnetic material to the target substance (nucleic acid or protein) is reversible throughout the use, as long as the nature and structure do not change. After sufficient washing, it can be recycled and reused. However, in practice, this issue needs to be considered separately, mainly based on the purpose of the magnetic beads used and the samples used.
Case 1: Magnetic beads are used to detect target substances
Generally speaking, if it is a magnetic bead used to detect a target substance, recycling is not recommended, because after the magnetic beads are used, it is unavoidable to carry the same residual substances, such as nucleic acids, proteins and other impurities, although washed. The method can remove most of the residue, but it can't be removed 100%. Even if one part of the substance remains, the repeated use of the magnetic beads after recycling may cause false positive detection. Especially in some high-sensitivity testing experiments, the magnetic beads cannot be reused because the source of each sample is different and the test results are very precise.
Case 2: Magnetic beads are used to enrich the single target substance of the raw material
If it is a magnetic bead for enriching the target substance, the product can be reused after recycling, in the case where the raw material is simply repeated, the product does not need to worry about the difference, the impurity is relatively small, and the production process is simple. For example, it is desirable to enrich the enzyme protein in the fermentation broth by magnetic bead method. Since all samples are the same process fermentation broth, the target product is the same enzyme protein, and there is no heterogeneous contamination in repeated use, and the magnetic beads can be recycled and reused. But this reuse can not be unlimited. Because each time, the impurities will occupy a certain functional group. As the number of repetitions increases, the effective sites of the magnetic beads become less and less, the adsorption efficiency decreases, and the purity of the purified product decreases. In severe cases, raw materials are wasted and qualified products cannot be obtained, so even if the magnetic beads can be reused, the number of repeated uses is limited.
Case 3: The structure or properties of the magnetic beads have changed.
In other experiments, some of the groups modified on the magnetic beads are lost with product rupture, and the functionality of the magnetic beads is also lost after use. If the magnetic bead structure is "magnetic nucleus-AB", after the target product C is intervened, the structure becomes "magnetic nucleus-ABC", after the elution, the chemical bond between the groups AB is broken, and the group B enters the elution with the target product C. The liquid becomes a structure of "magnetic core-A" and "BC". Due to the loss of functional groups, the magnetic beads can no longer be reused. This condition is common in the use of immunomagnetic beads. Therefore, before using the magnetic beads, you should understand the principle and use of the magnetic beads. This will change the surface of the magnetic beads and you will not be able to recycle the magnetic beads.
In general, recycling helps reduce costs, but as magnetic bead production technology continues to mature, the price of biomagnetic beads is gradually decreasing, and when recycling magnetic beads, continuous cleaning and other operations are required to ensure stability. Sex, the cost may be higher. As a standard magnetic bead manufacturer, Jiente Biotech will try to adjust the price of magnetic beads to a more reasonable range under the premise of ensuring the scale of production and product stability, and provide low-cost, high-quality biomagnetics for the majority of users. Beads. For more details, please contact the Senior Manager or visit the Genen Bio website ().
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