Food is inseparable from our lives. If the food contains hormones, it will cause great harm to our health. Therefore, Shanghai Ruijie Chromatography believes that people need to use liquid chromatography to detect the presence or absence of hormones in food.
For the determination of the hormone content in food, the most commonly used method is the liquid chromatography method. Here are some experts from this aspect of the analyzer to introduce you to its detailed operation method.
1, extract
Take a piece of food first, then take the edible part, knead them into a homogenate, and then take about 50g and put it in a 250mL conical flask. Add 20.00 μg/mL of methaqualone and 1.0 mL of stock solution, and then add 150 ml of a 1 to 1 mixture of methanol and acetone, then place on a shaker and shake for 30 minutes, then filter. The remaining residue was washed several times with a small amount of a mixture of methanol and acetone, and poured into a filtrate, which was placed in a rotary evaporator to evaporate the solution.
The remaining residue was dissolved in 20 ml of methanol, filtered from a funnel to a separatory funnel, and the funnel and contents were washed several times with 10 ml of a methanol solution, and the final washing liquid was poured into the filtrate. About 80 mL of a sodium acetate solution having a pH of 5.2 was added, and after uniformly mixing, it was extracted by shaking gently with shaking water three times, and they were combined and dehydrated with anhydrous sodium sulfate. The residue was dissolved in methanol, and a layer of supernatant from the upper layer of the solution was taken for analysis by liquid chromatography.
2, determination
10 μL of the sample solution for chromatographic detection was taken out, placed in a liquid chromatograph, and quantified by an internal standard method.
The above is a specific method of using the Shanghai Ruijie chromatograph to determine whether the food contains hormones. If you want to test, you can refer to the above introduction.
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