GAPDH Antibody Recommendation - Abbkine Cost-effective GAPDH Internal Reference Antibody Selection

In the Western Bloting experiment, there may be inaccurate determination of the total protein concentration, or the operational error between the samples produced when the protein sample is loaded before electrophoresis; these errors can be determined by measuring the internal parameters actually transferred to the membrane in each sample, such as The content of the internal reference GAPDH was corrected. GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is A key enzyme involved in glycolysis consisting of four 30-40 kDa subunits with a molecular weight of 146 kDa and a detection band of approximately 36 kDa. The GAPDH gene is expressed at high levels in almost all tissues and is widely used as an internal reference for Western blot protein standardization.

How to choose the right GAPDH antibody?

Although there are many choices of internal reference antibodies, they also need to follow certain principles and meet the practical application requirements. First, we need to consider the size of the protein of interest. We recommend that the internal reference should have a molecular weight difference of more than 5KD from the target protein to be detected. Therefore, you need to select the appropriate internal reference according to the molecular weight of the protein you are trying to detect, because the molecular weight of GAPDH is about 36 kD, therefore the internal reference antibody is not well suited for use in WB assays for detecting target proteins of 30 kD-40 kD size. In addition, there are certain differences in the selection of internal reference samples from different tissue sources. For example, in some cells, due to tissue hypoxia, diabetes and other factors, the expression of GAPDH is increased, which is not suitable for internal reference.

Because the molecular weight of the target protein was about 50kD, the previous β-Tubulin was not very suitable. I bought an Abbkine GAPDH antibody as an internal reference. The titer was a bit surprising. I used a 1:1000-fold initial dilution to think that the band was very Thick, and later diluted to 1:8000, while reducing the exposure time, the strip is still very clear. ——One client of the Life Science Institute of Wuhan University

In general, the more species types that can be detected by the internal reference antibody, the greater the choice in use; while the mouse-derived monoclonal antibodies generally have better specificity, stability, and potency than polyclonal. antibody. In addition, the titer of the antibody is also an important aspect to examine the cost performance of the antibody, which is equivalent to the dilution ratio in practical use. A high titer of 100 μl of GAPDH antibody (if the dilution ratio of WB assay is 1:5000, the final use solution is equivalent to 500 ml), 1 ml of GAPDH antibody with lower potency (if the dilution ratio of WB assay is 1:200) The final use solution is equivalent to 2ml). Therefore, when selecting antibodies, not only the amount of antibody needs to be considered, but also the titer of the antibody, that is, the recommended dilution ratio for different applications.

Why choose to recommend the Abbkine brand of GAPDH ?

There are many imported brands that supply GAPDH antibodies, such as Abcam, Santa Cruz, Cell Signaling (CST), Millipore, etc., which are related to GAPDH antibodies. However, because it is not a specialized label and internal reference antibody supplier, it is not the best choice for cost performance. For Abcam, for example, a 100μl GAPDH antibody is quoted at ¥4418; a CST 100μl GAPDH antibody is quoted at ¥2997.

In addition, some domestic suppliers can also provide sub-packaged GAPDH antibodies, but there are deficiencies in brand and quality assurance. The domestically packaged GAPDH body is insufficient in quality stability, and its valence is also lost after being packaged. Some GAPDH antibodies originating in domestic laboratories have better detection activity, but there are gaps in application types and it is difficult to control the stability between batches, and only a small amount of domestically produced antibodies are also available in China. Polyclonal antibody. If you want to buy a GAPDH internal reference antibody that is more cost-effective and also an imported brand, then Abbkine may be one of your important considerations. We recommend Abbkine's GAPHD monoclonal reference antibody Anti-GAPDH Monoclonal Antibody (2B5) (Cat. No. A01020).

The antibody monoclonal antibody has the clone number 2B5. Its antigen is a partial sequence of GAPDH protein with both protein specificity and species versatility. It has a wide range of detection species and can recognize GAPDH of human, mouse, rabbit, rat, monkey, dog, chicken, pig and sheep. Protein, also known as a truly conserved internal reference antibody. In addition, the titer of this antibody is very high, the recommended dilution ratio for WB experiments is 1:1000-1:10,000, and even customers have reported higher dilution ratios.

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Abbkine also offers a large package of 1ml of GAPDH internal reference antibody, which is ideal for routine internal validation or calibration, or for immunological experiments in cytoskeletal fluorescence localization. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during the use, please contact your local agent Ai Meijie Technology Co., Ltd. directly.

Product name and description	Brand	Item number	product manual
Anti-GAPDH Monoclonal Antibody (2B5)	Abbkine	A01020	Details
HRP Conjugated Anti-GAPDH Monoclonal Antibody (2B5)	Abbkine	A01025	Details

The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its regional agent in China, and will provide customers with the most cost-effective routine immunological reagents, such as A tag antibody, an internal reference antibody, a conventional primary antibody, and a full range of secondary antibodies, antibody detection substrates, and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to 801/802/803 or 027-84454412/4876 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.

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