New progress in stem cell culture manufacturing technology !
Stem cells are primitive cells that can survive for long periods of time and have the ability to self-reproduce and multi-directionalize, almost in all tissues. In recent years, with the deepening of research by scientists, stem cells have seen hope in the treatment of various diseases such as blood system diseases, nervous system diseases, cardiovascular diseases, autoimmune diseases, and endocrine diseases.
Stem cell technology is one of the most advanced and hottest directions in medical research. It has developed rapidly in recent years and has achieved exciting results. At the same time, scientists have made great achievements in the cultivation and manufacture of stem cells. Recently published in Cell. In a study by Stem Cell, scientists from the Massachusetts General Hospital developed a new program that could revolutionize the process of adult stem cell culture.
In this article, Xiaobian has carried out an inventory of new technologies or progress in stem cell manufacturing and sharing.
[1] SCTM: First developed method for producing adult stem cells
Doi:10.5966/sctm.2011-0022
Scientists at the University of Queensland in Australia have developed the first method of producing adult stem cells in the world. This research will profoundly affect patients with a series of serious diseases.
The study was conducted in collaboration with a number of research institutions, including the University of Queensland's Australian Institute of Bioengineering and Nanotechnology, led by Nicholas Fisk, a professor at the University of Queensland Clinical Research Center.
Mesenchymal stem cells (MSCs) can be used to repair bones and potentially repair other organs. This study reveals a new approach to producing mesenchymal stem cells.
Professor Fisk said, "We use a small molecule, SB431542, a transforming growth factor-β (TGF-β) pathway inhibitor, to induce embryonic stem cells for 10 days (to produce mesenchyme). Stem cells) are produced at a much faster rate than other studies reported in the literature. This technique can also be applied to less controversial induced pluripotent stem cells (iPSCs).
[2] Biofabrication: A major breakthrough in 3D printing of human embryonic stem cells
DOI: 10.1088/1758-5082/5/1/015013
In a new study, a team from Scotland first used a new three-dimensional printing technique to arrange human embryonic stem cells (hESCs). The results of the study were published on February 5, 2013. In the Biofabrication journal, the paper titled "Development of a valve-based cell printer for the formation of human embryonic stem cell spheroid aggregates". This breakthrough is expected to allow people to use hESCs to construct three-dimensional tissues and structures, thereby accelerating and improving the drug testing process.
In the field of biomanufacturing, the fabrication of three-dimensional tissues and organs by combining artificial solid structures and cells has made great progress in recent years. However, in most previous studies, animal cells were used to test different printing methods in order to fabricate these structures. .
Dr. Will Wenmiao Shu, co-author of the paper and researcher at Heriot-Watt University in Scotland, said, "As far as we know, this is the first time to print hESCs. Using hESCs to create three-dimensional structures will allow us to build more accurate human tissue models, and these people Tissue models are required for drug development and toxicity testing in vitro. Because most drug development is directed at human disease, the use of human tissue makes sense."
In the long term, this new method of printing may lay the foundation for the integration of hESCs into artificially constructed organs and tissues that are ready to be transplanted into patients with different diseases.
[3] Biofabrication: Handheld 3D "printing pen" can efficiently print human stem cells
Doi:10.1088/1758-5090/8/1/015019
Recently, in a research report published in the international magazine Biofabrication, researchers from Australia successfully used a hand-held 3D printing pen to successfully map human stem cells with high survival rate in free mode. The new device developed by the researchers can help surgeons perform personalized cartilage transplants during surgery.
The researchers pointed out that the use of hydrogel-type "bio-ink" to carry and support human stem cell growth, and the use of a lower light source to coagulate "bio-ink", the survival rate of stem cells transported by the pen will exceed 97%. This new type of 3D printing pen also greatly helps tissue engineering research, for example, it can print out cells layer by layer to build artificial tissue for transplantation.
However, in some cases, such as during cartilage repair, the precise geometric properties of the implant may not be accurately applied to the surgical procedure, which makes the preparation of the artificial cartilage tissue graft become Complex and difficult; the new print pen acts like a surgeon's hand and can accurately fill a custom-made stent or graft into a missing part of the patient's body. Researcher Professor Choong said that the development of this new type of device is the result of a joint effort between scientists and clinicians, and will bring unprecedented changes to improved research and patient care.
[4] Nature Materials: "squeezing" cells into stem cells
DOI: 10.1038/nmat4536
Scientists at the EPFL in Lausanne, Switzerland, have recently developed a new method to help cells become available stem cells. This approach involves the use of gels to "squeeze" cells, paving the way for large-scale production of stem cells for medical use.
Stem cells are currently at the forefront of modern medicine. They can be transformed into cells of different organs and are expected to provide new ways to treat a range of injuries and diseases. But producing the right type of stem cells in a standardized way remains a serious challenge. EPEL scientists have developed a gel that enhances the ability of cells to reprogram into stem cells by three-dimensional "squeezing" cell formation. The study, published online November 11, 2016 in the journal Nature Materials, also makes it easy to expand stem cell production and perform a wide range of applications on an industrial scale.
There are different types of stem cells, among which "medicinal pluripotent stem cells (iPSCs)" are particularly attractive. These stem cells are derived from mature, adult cells of gene reprogramming and appear as stem cells. iPSCs can be regenerated into a range of different cell types, such as liver, pancreas, lung, skin and other cells.
[5] Cell Stem Cell: Developed a method for long-term culture of adult stem cells in vitro
Doi:10.1016/j.stem.2016.05.012
In a new study, a new method developed by researchers from institutions such as the Massachusetts General Hospital (MGH) may trigger a revolution in adult stem cell culture. The researchers described obtaining airway stem cells from various tissue samples collected during daily treatment of lung disease and proliferating them. This method also appears to be applicable to several other tissues, such as the skin, the lining of the gastrointestinal tract, and the reproductive tract. The results of the study were published online June 16, 2016 in the journal Cell Stem Cell, entitled "Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells".
"This new approach opens up new avenues for the study of asthma or chronic obstructive pulmonary disease (COPD) and any other airway disease," said Jayaraj, author of the paper, MGH Reproductive Medicine Center, and associate professor of medicine at Harvard Medical School. Dr. Rajagopal said, “Although we have only been able to allow adult stem cells to proliferate for generations in the past, we are now able to grow enough adult stem cells for experimentation in several laboratories for several years. Our approach is also very simple and avoid The complexity of the previous culture system made it easier for many laboratories to adopt it."
[6] Nat Commun: Three stem cell manufacturing technologies are proven safe
Doi:10.1038/ncomms10536
In a new study, researchers from the Scripps Research Institute (TSRI) and the J. Craig Venter Institute (JCVI) confirmed Methods for making clinically used pluripotent stem cells are unlikely to deliver oncogene mutations to patients. The relevant research results were published in the journal Nature Communications on February 19, 2016, and the title of the paper is "Whole-genome mutational burden analysis of three pluripotency induction methods". This study is an important step in assessing the safety of rapidly developing stem cell therapies.
This new study focuses on the safety of using induced pluripotent stem cells (iPSCs) in patients. Because iPSCs are capable of differentiating into any type of cell in the body, they have the potential to repair damage caused by abrasions or diseases such as Parkinson's disease and multiple sclerosis.
"We want to know if reprogramming cells makes them prone to mutations," said Jeanne Loring, a professor of TSRI developmental neurobiology who led the new study with Professor Nicholas J. Schork, director of human biology at JCVI. The answer is 'no' ""
[7] Nature: Help! Scientists discover new ways to grow hematopoietic stem cells
DOI: 10.1038/nature17665
Researchers at the McMaster University's Stem Cell and Cancer Research Institute have made significant advances in understanding the stem cells of the human blood system, and they have discovered how a key protein allows these cells to better control and regeneration.
The study, recently published in the journal Nature, illustrates how a protein called Musashi-2 regulates the function and development of important hematopoietic stem cells.
The study found new strategies that can be used to control the growth of these stem cells. These cells can be used to treat a range of deadly diseases, but they are often in short supply.
The research's senior author, Kristin Hope, is a Principal Investigator at the Stem Cell and Cancer Institute and an Assistant Professor in the Department of Biochemistry and Biomedical Sciences at McMaster University. Other collaborators include researchers from the University of California, San Diego, the University of Toronto, and the University of Montreal.
Hope said the findings could have profound effects on thousands of patients suffering from a range of blood diseases, including leukemia, lymphoma, aplastic anemia, and sickle cell disease.
[8] PNAS: Scientists developed the first adult induced specific stem cells
On April 4th, a research team from the University of New South Wales (UNSW) published an article in the top journal PNAS, which was the first to develop a revolutionary stem cell repair technology. This technology can successfully re-inducing fat and bone cells into pluripotent stem cells, and is expected to be used in the treatment of human injuries including spinal injuries and fractures.
This technique is similar to the regeneration of limbs. Its most prominent achievement is the transformation of adult cells into induced immense stem cells (iMS cells), and iMS cells have the function of self-renewal and differentiation into many types of cells. This iMS cell can treat the human body damage caused by disease, aging or trauma, and will revolutionize the current state of regenerative medicine treatment of body damage.
The first induced specific adult stem cell (iMS): capable of self-renewal repair, differentiation into multiple types of cells.
According to the stage of development, stem cells can be divided into embryonic stem cells (ES cells, which have the ability to differentiate into intact individuals) and adult stem cells (somatic stem cells). Adult stem cells have the function of differentiating into specific cells and are not capable of differentiating into various cell types. The breakthrough in this latest technology published by PNAS is that induced specific stem cells can differentiate into many types of cells.
[9] PNAS: adult fat cells can differentiate into pluripotent stem cells, or can be used for tissue damage repair <br> News Read: New stem cell treatment using fat cells could repair any tissue in the body
For the first time, Australian scientists have reprogrammed adult bones or fat cells to obtain stem cells that can differentiate into any tissue, thereby repairing damaged tissues and organs.
Inspired by the phenomenon that lizards can regenerate limbs, these researchers have developed techniques that can return adult cells to the state of stem cells and gain the potential for division and multi-directional differentiation - pluripotent stem cells. This means that this part of the cell can repair damage to any part of the body: including the spinal cord, joints and muscles. The significance of this study is that there have been no previous reports of successful differentiation of adult stem cells into multiple types of tissues.
"This technology is a revolutionary advancement in stem cell therapy, and there has never been evidence that adult stem cells can differentiate directly into tissues." John Pimanda, Principal Investigator from the University of New South Wales, said. "We are currently investigating that adult fat cells can become inducible pluripotent stem cells through reprogramming techniques, thereby repairing tissue damage in mice. It is expected to enter clinical trials in 2017."
[10] Nature: A major breakthrough! First produced human haploid embryonic stem cells!
Doi:10.1038/nature17408
In a new study, researchers from the Hebrew University of Jerusalem, the University of Columbia Medical Center, and the New York Stem Cell Foundation Institute succeeded in producing a new type of embryonic stem cell that carries only a single copy of the human genome. Rather than two copies of the human genome normally found in normal stem cells. The relevant research results were published online in the Nature Journal on March 16, 2016, and the title of the paper is "Derivation and differentiation of haploid human embryonic stem cells".
The haploid embryonic stem cells described in this study are the first known human daughter cells capable of producing a single copy of the genome of a parental cell by cell division.
Human cells are considered to be diploid because they inherit two sets of chromosomes, for a total of 46 chromosomes, 23 of which are from the mother and 23 from the father. The only exceptions are germ cells (eggs and sperm), which are haploid cells and contain a set of chromosomes, 23 chromosomes. These haploid cells cannot produce more eggs and sperm by dividing.
Efforts to use human egg cells to produce embryonic stem cells have previously led to the production of diploid stem cells. In this study, the researchers promoted the division of unfertilized human eggs. They then labeled the DNA with a fluorescent dye to isolate these haploid embryonic stem cells, which were scattered among more diploid cells.
Beijing Baiou Bowei Biotechnology Co., Ltd. has set up a cell line, which is a center for cell lines. It is specialized in providing cell lines with low generation, short cycle and good activity. With a number of domestic and foreign research and development units, biomedicine, third-party testing institutions, research institutes have a good and stable long-term cooperative relationship! Welcome customers to inquire!
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Stem cells are primitive cells that can survive for long periods of time and have the ability to self-reproduce and multi-directionalize, almost in all tissues. In recent years, with the deepening of research by scientists, stem cells have seen hope in the treatment of various diseases such as blood system diseases, nervous system diseases, cardiovascular diseases, autoimmune diseases, and endocrine diseases.
Stem cell technology is one of the most advanced and hottest directions in medical research. It has developed rapidly in recent years and has achieved exciting results. At the same time, scientists have made great achievements in the cultivation and manufacture of stem cells. Recently published in Cell. In a study by Stem Cell, scientists from the Massachusetts General Hospital developed a new program that could revolutionize the process of adult stem cell culture.
In this article, Xiaobian has carried out an inventory of new technologies or progress in stem cell manufacturing and sharing.
[1] SCTM: First developed method for producing adult stem cells
Doi:10.5966/sctm.2011-0022
Scientists at the University of Queensland in Australia have developed the first method of producing adult stem cells in the world. This research will profoundly affect patients with a series of serious diseases.
The study was conducted in collaboration with a number of research institutions, including the University of Queensland's Australian Institute of Bioengineering and Nanotechnology, led by Nicholas Fisk, a professor at the University of Queensland Clinical Research Center.
Mesenchymal stem cells (MSCs) can be used to repair bones and potentially repair other organs. This study reveals a new approach to producing mesenchymal stem cells.
Professor Fisk said, "We use a small molecule, SB431542, a transforming growth factor-β (TGF-β) pathway inhibitor, to induce embryonic stem cells for 10 days (to produce mesenchyme). Stem cells) are produced at a much faster rate than other studies reported in the literature. This technique can also be applied to less controversial induced pluripotent stem cells (iPSCs).
[2] Biofabrication: A major breakthrough in 3D printing of human embryonic stem cells
DOI: 10.1088/1758-5082/5/1/015013
In a new study, a team from Scotland first used a new three-dimensional printing technique to arrange human embryonic stem cells (hESCs). The results of the study were published on February 5, 2013. In the Biofabrication journal, the paper titled "Development of a valve-based cell printer for the formation of human embryonic stem cell spheroid aggregates". This breakthrough is expected to allow people to use hESCs to construct three-dimensional tissues and structures, thereby accelerating and improving the drug testing process.
In the field of biomanufacturing, the fabrication of three-dimensional tissues and organs by combining artificial solid structures and cells has made great progress in recent years. However, in most previous studies, animal cells were used to test different printing methods in order to fabricate these structures. .
Dr. Will Wenmiao Shu, co-author of the paper and researcher at Heriot-Watt University in Scotland, said, "As far as we know, this is the first time to print hESCs. Using hESCs to create three-dimensional structures will allow us to build more accurate human tissue models, and these people Tissue models are required for drug development and toxicity testing in vitro. Because most drug development is directed at human disease, the use of human tissue makes sense."
In the long term, this new method of printing may lay the foundation for the integration of hESCs into artificially constructed organs and tissues that are ready to be transplanted into patients with different diseases.
[3] Biofabrication: Handheld 3D "printing pen" can efficiently print human stem cells
Doi:10.1088/1758-5090/8/1/015019
Recently, in a research report published in the international magazine Biofabrication, researchers from Australia successfully used a hand-held 3D printing pen to successfully map human stem cells with high survival rate in free mode. The new device developed by the researchers can help surgeons perform personalized cartilage transplants during surgery.
The researchers pointed out that the use of hydrogel-type "bio-ink" to carry and support human stem cell growth, and the use of a lower light source to coagulate "bio-ink", the survival rate of stem cells transported by the pen will exceed 97%. This new type of 3D printing pen also greatly helps tissue engineering research, for example, it can print out cells layer by layer to build artificial tissue for transplantation.
However, in some cases, such as during cartilage repair, the precise geometric properties of the implant may not be accurately applied to the surgical procedure, which makes the preparation of the artificial cartilage tissue graft become Complex and difficult; the new print pen acts like a surgeon's hand and can accurately fill a custom-made stent or graft into a missing part of the patient's body. Researcher Professor Choong said that the development of this new type of device is the result of a joint effort between scientists and clinicians, and will bring unprecedented changes to improved research and patient care.
[4] Nature Materials: "squeezing" cells into stem cells
DOI: 10.1038/nmat4536
Scientists at the EPFL in Lausanne, Switzerland, have recently developed a new method to help cells become available stem cells. This approach involves the use of gels to "squeeze" cells, paving the way for large-scale production of stem cells for medical use.
Stem cells are currently at the forefront of modern medicine. They can be transformed into cells of different organs and are expected to provide new ways to treat a range of injuries and diseases. But producing the right type of stem cells in a standardized way remains a serious challenge. EPEL scientists have developed a gel that enhances the ability of cells to reprogram into stem cells by three-dimensional "squeezing" cell formation. The study, published online November 11, 2016 in the journal Nature Materials, also makes it easy to expand stem cell production and perform a wide range of applications on an industrial scale.
There are different types of stem cells, among which "medicinal pluripotent stem cells (iPSCs)" are particularly attractive. These stem cells are derived from mature, adult cells of gene reprogramming and appear as stem cells. iPSCs can be regenerated into a range of different cell types, such as liver, pancreas, lung, skin and other cells.
[5] Cell Stem Cell: Developed a method for long-term culture of adult stem cells in vitro
Doi:10.1016/j.stem.2016.05.012
In a new study, a new method developed by researchers from institutions such as the Massachusetts General Hospital (MGH) may trigger a revolution in adult stem cell culture. The researchers described obtaining airway stem cells from various tissue samples collected during daily treatment of lung disease and proliferating them. This method also appears to be applicable to several other tissues, such as the skin, the lining of the gastrointestinal tract, and the reproductive tract. The results of the study were published online June 16, 2016 in the journal Cell Stem Cell, entitled "Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells".
"This new approach opens up new avenues for the study of asthma or chronic obstructive pulmonary disease (COPD) and any other airway disease," said Jayaraj, author of the paper, MGH Reproductive Medicine Center, and associate professor of medicine at Harvard Medical School. Dr. Rajagopal said, “Although we have only been able to allow adult stem cells to proliferate for generations in the past, we are now able to grow enough adult stem cells for experimentation in several laboratories for several years. Our approach is also very simple and avoid The complexity of the previous culture system made it easier for many laboratories to adopt it."
[6] Nat Commun: Three stem cell manufacturing technologies are proven safe
Doi:10.1038/ncomms10536
In a new study, researchers from the Scripps Research Institute (TSRI) and the J. Craig Venter Institute (JCVI) confirmed Methods for making clinically used pluripotent stem cells are unlikely to deliver oncogene mutations to patients. The relevant research results were published in the journal Nature Communications on February 19, 2016, and the title of the paper is "Whole-genome mutational burden analysis of three pluripotency induction methods". This study is an important step in assessing the safety of rapidly developing stem cell therapies.
This new study focuses on the safety of using induced pluripotent stem cells (iPSCs) in patients. Because iPSCs are capable of differentiating into any type of cell in the body, they have the potential to repair damage caused by abrasions or diseases such as Parkinson's disease and multiple sclerosis.
"We want to know if reprogramming cells makes them prone to mutations," said Jeanne Loring, a professor of TSRI developmental neurobiology who led the new study with Professor Nicholas J. Schork, director of human biology at JCVI. The answer is 'no' ""
[7] Nature: Help! Scientists discover new ways to grow hematopoietic stem cells
DOI: 10.1038/nature17665
Researchers at the McMaster University's Stem Cell and Cancer Research Institute have made significant advances in understanding the stem cells of the human blood system, and they have discovered how a key protein allows these cells to better control and regeneration.
The study, recently published in the journal Nature, illustrates how a protein called Musashi-2 regulates the function and development of important hematopoietic stem cells.
The study found new strategies that can be used to control the growth of these stem cells. These cells can be used to treat a range of deadly diseases, but they are often in short supply.
The research's senior author, Kristin Hope, is a Principal Investigator at the Stem Cell and Cancer Institute and an Assistant Professor in the Department of Biochemistry and Biomedical Sciences at McMaster University. Other collaborators include researchers from the University of California, San Diego, the University of Toronto, and the University of Montreal.
Hope said the findings could have profound effects on thousands of patients suffering from a range of blood diseases, including leukemia, lymphoma, aplastic anemia, and sickle cell disease.
[8] PNAS: Scientists developed the first adult induced specific stem cells
On April 4th, a research team from the University of New South Wales (UNSW) published an article in the top journal PNAS, which was the first to develop a revolutionary stem cell repair technology. This technology can successfully re-inducing fat and bone cells into pluripotent stem cells, and is expected to be used in the treatment of human injuries including spinal injuries and fractures.
This technique is similar to the regeneration of limbs. Its most prominent achievement is the transformation of adult cells into induced immense stem cells (iMS cells), and iMS cells have the function of self-renewal and differentiation into many types of cells. This iMS cell can treat the human body damage caused by disease, aging or trauma, and will revolutionize the current state of regenerative medicine treatment of body damage.
The first induced specific adult stem cell (iMS): capable of self-renewal repair, differentiation into multiple types of cells.
According to the stage of development, stem cells can be divided into embryonic stem cells (ES cells, which have the ability to differentiate into intact individuals) and adult stem cells (somatic stem cells). Adult stem cells have the function of differentiating into specific cells and are not capable of differentiating into various cell types. The breakthrough in this latest technology published by PNAS is that induced specific stem cells can differentiate into many types of cells.
[9] PNAS: adult fat cells can differentiate into pluripotent stem cells, or can be used for tissue damage repair <br> News Read: New stem cell treatment using fat cells could repair any tissue in the body
For the first time, Australian scientists have reprogrammed adult bones or fat cells to obtain stem cells that can differentiate into any tissue, thereby repairing damaged tissues and organs.
Inspired by the phenomenon that lizards can regenerate limbs, these researchers have developed techniques that can return adult cells to the state of stem cells and gain the potential for division and multi-directional differentiation - pluripotent stem cells. This means that this part of the cell can repair damage to any part of the body: including the spinal cord, joints and muscles. The significance of this study is that there have been no previous reports of successful differentiation of adult stem cells into multiple types of tissues.
"This technology is a revolutionary advancement in stem cell therapy, and there has never been evidence that adult stem cells can differentiate directly into tissues." John Pimanda, Principal Investigator from the University of New South Wales, said. "We are currently investigating that adult fat cells can become inducible pluripotent stem cells through reprogramming techniques, thereby repairing tissue damage in mice. It is expected to enter clinical trials in 2017."
[10] Nature: A major breakthrough! First produced human haploid embryonic stem cells!
Doi:10.1038/nature17408
In a new study, researchers from the Hebrew University of Jerusalem, the University of Columbia Medical Center, and the New York Stem Cell Foundation Institute succeeded in producing a new type of embryonic stem cell that carries only a single copy of the human genome. Rather than two copies of the human genome normally found in normal stem cells. The relevant research results were published online in the Nature Journal on March 16, 2016, and the title of the paper is "Derivation and differentiation of haploid human embryonic stem cells".
The haploid embryonic stem cells described in this study are the first known human daughter cells capable of producing a single copy of the genome of a parental cell by cell division.
Human cells are considered to be diploid because they inherit two sets of chromosomes, for a total of 46 chromosomes, 23 of which are from the mother and 23 from the father. The only exceptions are germ cells (eggs and sperm), which are haploid cells and contain a set of chromosomes, 23 chromosomes. These haploid cells cannot produce more eggs and sperm by dividing.
Efforts to use human egg cells to produce embryonic stem cells have previously led to the production of diploid stem cells. In this study, the researchers promoted the division of unfertilized human eggs. They then labeled the DNA with a fluorescent dye to isolate these haploid embryonic stem cells, which were scattered among more diploid cells.
Beijing Baiou Bowei Biotechnology Co., Ltd. has set up a cell line, which is a center for cell lines. It is specialized in providing cell lines with low generation, short cycle and good activity. With a number of domestic and foreign research and development units, biomedicine, third-party testing institutions, research institutes have a good and stable long-term cooperative relationship! Welcome customers to inquire!
Http://
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